Advances in Enzymology and Related Areas of Molecular by Alton Meister

By Alton Meister

A Unifying version of the Thermodynamics of Formation of Dehydrogenase-Ligand Complexes (H. Fisher).

Sorbitol Dehydrogenase (J. Jeffery and H. Jornvall).

Molecular measurement choice of Enzymes via Radiation Inactivation (E. Kempner).

Calcineurin (C. Klee, et al).

The habit and value of Slow-Binding Enzyme Inhibitors (J. Morrison and C. Walsh).

ADP-Ribosylation of Guanyl Nucleotide-Binding Regulatory Proteins by way of Bacterial pollution (J. Moss and M. Vaughan).

Kinetics of Substrate response in the course of Irreversible amendment of Enzyme job (C. Tsou).

The Dynamics of DNA Polymerase-Catalyzed Reactions (V. Mizrahi and S. Benkovic).

writer Index.

topic Index.

Cummulative Indexes, Vols 1-61.Content:

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Extra info for Advances in Enzymology and Related Areas of Molecular Biology, Volume 61

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7 and 8. We will now find it convenient to focus on the closely related changes in thermodynamic parameters caused by such shifts as they would express themselves in isothermal measurements (25 and 15"C), since such measurements are available for many more systems than the handful of systems portrayed in Fig. 4. In Fig. 11 we have plotted a number of thermodynamic parameters that would be observed for a given enzyme form at the conventional measurement temperature (25°C) as a function of the intrinsic TOvalue of that form, the only variable in our model.

2. This arrangement permits us to compare the results of constant temperature binding studies (such as those shown in Fig. 4) with those predicted by the model in a convenient manner. The two heavy solid lines crossing the curves in Fig. 9 indicate the changes in A H " between the enzyme forms for ternary complex formation at 15 and 25°C. While Fig. 9 necessarily 26 HARVEY F. FISHER portrays only a single example of the general model proposed here, and is based on a somewhat arbitrary choice of To parameters, it can nevertheless serve as a basis for exploring the range of phenomena that can be produced by the model and set some limits to the scope of the theory.

Con- 52 JONATHAN JEFFERY AND HANS JORNVALL sumption of sorbitol can also be a cause of functional gastrointestinal complaints in idiosyncratically sensitive adults. 0-g sorbitol/piece (90-92). For adults who do not use sorbitol as a sugar substitute, the usual daily intake of sorbitol commonly ranges from <2 g (ordinary mixed diets) to about 16 g (some vegetarian diets). 8 kcal/g (16 kJ/g) (93). Therefore, effects other than direct contribution to energy balance determine the dietary significance of sorbitol.

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