Agrobacterium Protocols, Second Edition: Volume II (Methods by Kan Wang

By Kan Wang

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Additional info for Agrobacterium Protocols, Second Edition: Volume II (Methods in Molecular Biology Vol 344)

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21. 22. 23. 24. 25. 26. 27. Msikita et al. Scientific Meeting of the Cassava Biotechnology Network. M. , CIAT Working Document 123, 222–228. , and Marks, J. (2002) Influence of explant source, and light on efficiency of Agrobacterium-mediated transformation of cassava. Fifth International Meeting of the Cassava Biotechnology Network. St. , USA. November 4–9. , and Fauquet, C. (1997) An efficient mass propagation system for cassava (Manihot esculenta Crantz) based on nodal explants and auxiliary bud-derived meristems.

In this paper, transformation efficiency refers to the number of PCR amplified transgene-confirmed positive plants per total number of explants inoculated (23). Cassava (Manihot esculenta Crantz) 23 Acknowledgment Weston Msikita and Uzoma Ihemere contributed equally to this document. References 1. , and David, S. (2000) The transference of apomixis genes from Manihot neusana Nassar to cassava, M. esculenta Crantz. Hereditas 132, 167–170. 2. A. (1995) Development and selection for apomixis in cassava Manihot esculenta Crantz.

And Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol. 15, 473–497. 7. , and Muthukrishnan, S. (1998) Induction of thaumatin-like proteins (TLPs) in Rhizoctonia solani-infected rice and characterization of two new cDNA clones. Plant Physiol. 100, 1503–1507. 8. A. (2000) Disease response of carrot and carrot somaclones to Alternaria dauci. Plant Path. 49, 57–67. 9. C. (1998) A small-scale procedure for extracting nucleic acids from woody plants infected with various phytopathogens for PCR assay.

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