Animal Cell Electroporation and Electrofusion Protocols by Jac A. Nickoloff

By Jac A. Nickoloff

Harvard college, Boston, Massachusetts. guide of protocols within the electroporation of proteins and DNA into fish, insect, and mammalian cells. For researchers. Plastic comb spiral binding. sixty five individuals, 28 U.S.

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Extra resources for Animal Cell Electroporation and Electrofusion Protocols (Methods in Molecular Biology)

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T. Kubiniec, D. A. Stenger, H. Liang, and N. G. Stoicheva. The work was supported by a grant GM 30969 from the National Institutes of Health. References 1. Abidor, I. , Arakelyan, V. , Chernomordik, L. , Chizmadzhev, Y. , Pastushenko, V. , and Tarsevich, M R. (1979) Electrrc breakdown of bilayer membranes. I. The main experimental facts and their qualitative discussion Bioelectrochem. Bioenerg. 6,37-52. 2. Zhelev, D. V. and Needham, D. (1993) Tension-stabilized pores in gaint vesicles: determination of pore size and pore line tension.

Square- Wave Generators Square-wave pulses appearto have advantagesfor certain applications, such as transfection of mammalian cell lines and plant protoplasts, though no generalization can be made. Each cell line needs to be individually investigated to determine whether use of square-wave pulses would be advantageous. In general, square waves do not appear to result in higher transformation yields for bacteria, although there are some protocols that give good results (6; Xing Xin, Texas Heart Institute, personal communication).

5 a Number of cells showing 2 1 chromosomal rearrangement. Each pomt represents the mean of two independent experrments One hundred metaphase spreads were analyzed for each experrment. 9. d Electroporatron of the storage buffer alone (1 e , the PvuII shrppmg buffer without PvuII). 4. Notes 1. Electroporation can be used to mtroduce a variety of proteins mto cells, including monoclonal antibodies (MAb) (22,23), functional enzymes (24), other endonucleases in addition to restriction enzymes (25), and a host of other proteins and protein products (26-28).

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