Nucleic Acid Polymerases by Katsuhiko S. Murakami, Michael A. Trakselis

By Katsuhiko S. Murakami, Michael A. Trakselis

This booklet presents a evaluate of the multitude of nucleic acid polymerases, together with DNA and RNA polymerases from Archea, micro organism and Eukaryota, mitochondrial and viral polymerases, and different really expert polymerases equivalent to telomerase, template-independent terminal nucleotidyl transferase and RNA self-replication ribozyme. even supposing many books disguise numerous varieties of polymerases, no e-book thus far has tried to catalog all nucleic acid polymerases. The aim of this booklet is to be the pinnacle reference paintings for postgraduate scholars, postdocs, and precept investigators who examine polymerases of all kinds. In different phrases, this ebook is for polymerase fanatics by way of polymerase fans.

Nucleic acid polymerases play a primary position in genome replication, upkeep, gene expression and legislation. all through evolution those enzymes were pivotal in reworking lifestyles in the direction of RNA self-replicating platforms in addition to into extra good DNA genomes. those enzymes are more often than not tremendous effective and exact in RNA transcription and DNA replication and proportion universal kinetic and structural positive aspects. How catalysis could be so amazingly speedy with no lack of specificity is a question that has intrigued researchers for over 60 years. definite really expert polymerases that play a severe function in mobile metabolism are used for various biotechnological purposes and are accordingly an important instrument for research.

Show description

Read Online or Download Nucleic Acid Polymerases PDF

Best molecular biology books

Oxford Dictionary of Biochemistry and Molecular Biology

The Oxfоrd Dictionary of Biochemistry and Molecular Biology offers a finished survey of present biochemistry and molecular biology. during the last few years, the language of Biochemistry and Molecular Biology has multiplied greatly to the level that few scientists can anticipate to be acquainted with all facets of it.

Principles and Technical Aspects of PCR Amplification

Kary Mullis used to be presented a Nobel Prize for inventing the PCR approach greater than 15 years in the past in 1993. on the grounds that its "discovery", a number of variations and diversifications of the traditional PCR approach were defined, with a lot of those diversifications and adaptations at present getting used in medical, diagnostic and educational laboratories the world over.

Biological thermodynamics

Organic Thermodynamics presents an creation to the research of strength variations for college kids of the organic sciences. Donald Haynie makes use of a casual writing kind to introduce this middle topic in a way that may entice biology and biochemistry undergraduate scholars. The emphasis of the textual content is put on knowing uncomplicated options and constructing problem-solving abilities during the textual content.

Textbook of Cell Signalling in Cancer: An Educational Approach

This booklet offers a simplified, but complete, evaluation of the signalling pathways working among and inside of cells, as a way to support more youthful oncologists locate their approach within the labyrinth of signalling pathways and within the multitude of indications and sign receptors, transducers and effectors that give a contribution to oncogenesis.

Extra info for Nucleic Acid Polymerases

Sample text

Mol Cell Biol 21(14):4495–4504. 2001 Ferrari M, Lucchini G, Plevani P, Foiani M (1996) Phosphorylation of the DNA polymerase alphaprimase B subunit is dependent on its association with the p180 polypeptide. J Biol Chem 271 (15):8661–8666 Fien K, Cho YS, Lee JK, Raychaudhuri S, Tappin I, Hurwitz J (2004) Primer utilization by DNA polymerase alpha-primase is influenced by its interaction with Mcm10p. J Biol Chem 279 (16):16144–16153. M400142200, M400142200 [pii] Foiani M, Santocanale C, Plevani P, Lucchini G (1989) A single essential gene, PRI2, encodes the large subunit of DNA primase in Saccharomyces cerevisiae.

Pol ε polymerase and exonuclease activities are reduced in subassemblies lacking the C and D subunits, relative to the holoenzyme form (Aksenova et al. 2010). 4 A Highly Accurate Polymerase Pol ε is perhaps the most accurate eukaryotic DNA polymerase. Purified calf thymus Pol ε base substitution error rates determined in vitro ranged from 1/30,000 to 1/400,000 mutations per nucleotide synthesized, lower than either Pol δ or Pol α (Thomas et al. 1991). The S. c. Pol ε holoenzyme exhibits very low base substitution and single-base deletion error rates, 2 Â 10À5 and 5 Â 10À7 (Shcherbakova et al.

Doi:10. M108546200 Ducoux M, Urbach S, Baldacci G, Hubscher U, Koundrioukoff S, Christensen J, Hughes P (2001) Mediation of proliferating cell nuclear antigen (PCNA)-dependent DNA replication through a conserved p21(Cip1)-like PCNA-binding motif present in the third subunit of human DNA polymerase delta. J Biol Chem 276(52):49258–49266. M106990200, M106990200 [pii] Eckert KA, Kunkel TA (1993) Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH.

Download PDF sample

Rated 4.26 of 5 – based on 29 votes